The successful candidate will participate in a project aimed at a proteomic analysis of the Malaria parasite, Plasmodium falciparum (see Lasonder et al., Nature 419, 537). Our current analysis focuses on the identification and characterization of complexes that regulate transcription during the complex life cycle of the parasite with an emphasis on factors involved in antigenic variation.

Doctorate
The ideal candidate will have a PhD in Molecular Biology, Biochemistry, Mass Spectrometry or related field. (S)he must be able to demonstrate a firm understanding of gene expression regulation and biochemistry/protein purification. The candidates must also have ample experience in protein purification and/or mass spectrometry. Previous experience in TAP-tagging and cell culture is beneficial, but not mandatory.

Radboud University Nijmegen Faculteit der Natuurwetenschappen, Wiskunde en Informatica

Strategically located in Europe, Radboud University Nijmegen is one of the leading academic communities in the Netherlands. A place with a personal touch, where top-flight education and research take place on a beautiful green campus in modern buildings with state-of-art facilities.

The last five years have seen extraordinary advances in genomics through technological developments. First and foremost, the complete sequence of the human genome and that of the major genetic model organisms has been elucidated. Second, techniques to hybridise experimentally isolated nucleic acids to micrometer size spots of defined DNA probes immobilised on glass chips commonly known as ‘DNA chips’ have allowed unprecedented rapid data acquisition. Third, the development of high specificity affinity purification strategies that allow purification to homogeneity of low abundance protein complexes and subsequent analyses by liquid chromatography coupled mass spectrometry has been refined. The fourth advance occurred in bioinformatics, delivering whole suits of software to exploit, integrate and mine the avalanche of data. The Stunnenberg group has pioneered an experimental approach that couples immunoprecipitation of proteins cross-linked to their native chromosomal sites of action to DNA chips (ChIP-on-chip). The applications of this experimental approach are manifold and it is beyond doubt that most members of the theme will benefit from this novel functional chip. For example to directly identify target promoters of transcription factors or to globally examine changes in target site occupancy as a function of time, exogenous stimuli and disease condition and to distinguish indirect from direct effects as a function of chromosome structure and intracellular signalling. Here high accuracy mass spectrometry, another strength of the sub-theme, comes in as it allows direct determination of all peptides within one sample - provided that the virtual peptides are represented in the database.

Estimated maximum salary per month: eur 3000 - 3500
Salaryscale 10.
Maximum salary amount in Euro's a month 3472
Employment basis: Temporary for specified period
Duration of the contract: A period of 2 years
Maximum hours per week: 40

Additional information about the vacancy can be obtained from:

Henk Stunnenberg
Telephone number: +31-24-3610524
E-mail address:

Henk Stunnenberg
Telephone number: +31-24-3610524
E-mail address:

 
Or additional information can be obtained through one of the following links.

You can apply for this job before 27-01-2006 by sending your application to:

When applying for this job always mention the vacancynumber 62.79.05.