In vivo CD40-targeted gene transfer to cutaneous dendritic cells for anti-tumor T cell priming: pre-clinical validation and clinical implementation

We are looking for an enthousiastic and motivated candidate for a Post-doc position in the immunotherapy division of the department of Medical Oncology. Our division is embedded in the Laboratory of Experimental Pathology, soon to be a part of the newly built VUmc Cancer Center.

Research in our division focuses on Dendritic Cells (DC) and their role in the generation of tumor-specific T cell immunity with an emphasis on the in vivo modulation of DC for tumor immunotherapeutic purposes through adenovirus-mediated genetic targeting and local cytokine administration.

The Post-doc will work on a project funded by the Dutch Cancer Society (KWF). He/she will develop and evaluate CD40-targeted adenoviral vectors as a tumor vaccine vehicle targeted to dendritic cells in vivo. The project has a strong translational character. The preclinical experiments planned in the first two years are focused and specifically geared towards proof of principle to facilitate clinical implementation.

Doctorate
The post-doc requested should have a profound theoretical and technical knowledge in the field of immunology. This project proposal features a number of in vitro and in vivo techniques including culture of mouse and human dendritic cells, T cells, and skin explants, generation, production, and purification of fusion proteins, complemented by various read-outs including MLRs, CTL ELISPOT, (tetramer-) flowcytometric, and immuno-histochemical analyses, Western blotting and ELISA assays, as well as in vivo mouse tumor induction and monitoring. Candidates with previous experience with mouse tumor models are preferred.

- Teaching & Research (Scientific expertise: Health)

VU Medical Center

Duration of the contract: four years
Maximum hours per week: 36

Additional information about the vacancy can be obtained from:

dr. Tanja de Gruijl
Telephone number: 020-4444063
E-mail address:

prof. dr. Rik Scheper
Telephone number: 020-4444031
E-mail address:

 
Or additional information can be obtained through one of the following links.

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When applying for this job always mention the vacancynumber AT/vumc/2006/01.